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By Норман Роквелл

Замечательный альбом репродукций американского художника Нормана Роквела. Почуствуйте дух старой Америки 20-50-х годов прошлого века. Посмотрите какие живые и открытие лица!

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HSV-TK imaging 38 Herpes will be performed using positron emission tomography (PET) and 124-I labeled FIAU (2'-fluoro-1-beta-D-arabinofuranosyl-5-iodo-uracil); we have previously demonstrated that [124I]-FIAU PET imaging of HSV-TK activity following retroviral and adenoviral (ADV-tk) transduction is feasible, selective and quantitative. The preclinical studies in this proposal will: 1) extend these studies and demonstrate selective vector imaging, 2) provide a comparison between mHSV1 (G207 and R7020) and retroviral (DCSV and SFG) vectors in appropriate experimental animal models, and 3) provide imaging data that could support and justify the initiation of clinical trials.

An understanding of the mechanisms by which DNA is replicated and mutations evolve will be of value for future designs of new antiviral drugs and treatments. DNA polymerase (pol) is the pivotal enzyme involved in DNA replication. It plays the central role controlling the mutation rate by two major steps: selection of the correct nucleotides to be incorporated into the growing primer terminus, and proofreading or editing of the incorrectly incorporated nucleotides. Numerous mutagenesis and kinetic studies of a variety of Pols have already been conducted in vitro to reveal the mechanisms controlling the fidelity of DNA replication.

Where both types are expressed by the same cell the net cellular response triggered by co-ligation is thought to depend on the ratio of expression of the two opposing signals. We will use wildtype and FcgR gene knockout mice to test the premise that viral antigen:antibody complexes via co-ligation favor negative FcgR signaling, and so suppress herpes stromal keratitis development. Our studies will provide new information on the expression and function of FcgammaRs in ocular tissue and may lead to novel strategies for controlling corneal inflammation.

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